Spatial Analysis of Phospholipids in Insect Models by Positive Ionization Mode Matrix-Assisted Laser Desorption Ionization Mass Spectrometric Imaging

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Imaging (MALDI-MSI) has developed as a useful tool in generating comprehensive metabolite profiles along with spatial information model organisms. The ability of MALDI-MSI to generate situ whole organisms or specific tissue sections serves an efficient alternative solvent extraction protocols, especially for the identification biomolecules that are unstable under conditions. Herein, we have utilized spatially profile various classes lipids two organisms, Drosophila melanogaster (fruit fly), and Tribolium castaneum (red flour beetle). Five different phospholipids were imaged positive ionization mode both insect species. Confirmation m/z assignment selected was performed using on-tissue MS/MS fragmentation.